CD40 Antikörper

Produktnummer ABIN319728

Dieses Anti-CD40-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von CD40 in FACS, IP und IHC (fro). Geeignet für Human.

Kurzübersicht für CD40 Antikörper (ABIN319728)

Target: CD40

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD40 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: LOB7-6

Produktdetails anti-CD40 Antikörper

Spezifität: This antibody recognises the CD40 cell surface antigen.

Aufreinigung: Affinity chromatography on Protein A.

Immunogen: Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Flow Cytometry: 20 μg/mL, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on Frozen sections: 1-10 μg/mL. Immunohistochemistry on Paraffin sections: 1-10 μg/mL. This product requires protein digestion pre-treatment of paraffin embedded sections prior to staining eg. pronase or trypsin. Recommended Positive Control: Human Tonsil. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Tris buffered saline containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu CD40

Target: CD40

Andere Bezeichnung: CD40

Hintergrund: CD40 is a 48kD glycoprotein expressed by B lymphocytes and weakly by some monocytes. CD40 is involved in the process of B cell selection in germinal centres, and is vital in T cell-B cell interactions.Synonyms: B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40, TNFRSF5, Tumor necrosis factor receptor superfamily member 5

Gen-ID: 958

NCBI Accession: NP_001241

UniProt: P25942

Pathways: NF-kappaB Signalweg, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints